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Histology Department Information: Difference between revisions
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Histology Department Information (view source)
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'''<big>Anatomical Histopathology operates Monday to Friday 0800-1700</big>''' | '''<big>Anatomical Histopathology operates Monday to Friday 0800-1700</big>''' | ||
Process all routine histology, immunohistochemistry, and frozen sectioning in-house. Specialized testing is sent to external laboratories as indicated below. | |||
== Histology == | == Histology == | ||
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If a sample is required to be split for testing, ensure all tests are written on the request from clearly. Once CytoRich Red has been added to a sample, no other testing can be completed. | If a sample is required to be split for testing, ensure all tests are written on the request from clearly. Once CytoRich Red has been added to a sample, no other testing can be completed. | ||
Contact SCL Dunedin for enquiries and results for cytology specimens 03 477 6981 | Contact SCL Dunedin for enquiries and results for cytology specimens: 03 477 6981 | ||
== Cytogenetics == | == Cytogenetics == | ||
Cytogenetics testing is completed by Wellington Regional Genetics Laboratory. WRGL offers tissue culture, karyotyping, QF-PCR, DNA extraction and frozen sample storage for postnatal and post mortem tissue samples where chromosomal or genetic abnormalities are suspected (http://www.wellingtongenetics.co.nz/). Hastings Hospital laboratory is responsible for transporting the specimen to WRGL. | Cytogenetics testing is completed by Wellington Regional Genetics Laboratory. WRGL offers tissue culture, karyotyping, QF-PCR, DNA extraction and frozen sample storage for postnatal and post mortem tissue samples where chromosomal or genetic abnormalities are suspected (http://www.wellingtongenetics.co.nz/). Hastings Hospital laboratory is responsible for transporting the specimen to WRGL. | ||
For any sample requiring cytogenetic studies please clearly write on the request form the gestational age, site of biopsy, and "Cytogenetics Testing". Send the sample immediately to the laboratory. If the sample is also requiring routine histology, ensure | For any sample requiring cytogenetic studies please clearly write on the request form the gestational age, site of biopsy, and "Cytogenetics Testing". Send the sample immediately to the laboratory. If the sample is also requiring routine histology, ensure the sample for cytogenetics is taken <u>before</u> any formalin is added. Send both samples together to the laboratory. | ||
If further information is required please contact the Histology department (EXT 2636) or contact Wellington Regional Genetics Laboratory 04 918 6967. | |||
'''The laboratory staff are not responsible for taking a biopsy for cytogenetics. This is to be completed by the requesting clinician.''' | '''The laboratory staff are not responsible for taking a biopsy for cytogenetics. This is to be completed by the requesting clinician.''' | ||
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'''For Tissue Karyotype/Cytogenetic studies sample requirements are:''' | '''For Tissue Karyotype/Cytogenetic studies sample requirements are:''' | ||
'''Miscarriage (<20 weeks)''': Products of Conception including Chorionic villi, fetal limb, umbilical cord, collected in a sterile yellow lid container. Add sterile saline if transport is likely to delayed. | '''Miscarriage (<20 weeks)''': Products of Conception including Chorionic villi, fetal limb, umbilical cord, collected in a sterile yellow lid container. Add sterile saline if transport is likely to be delayed. | ||
'''IUD, Stillbirth, perinatal death (>20weeks)''', '''Other:''' | '''IUD, Stillbirth, perinatal death (>20weeks)''', '''Other:''' | ||
Skin – 1 cm full thickness, cartilage, spleen, umbilical cord (1 cm from near site on insertion). Place in sterile yellow lid container. Add sterile saline if transport is likely to delayed. | Skin – 1 cm full thickness, cartilage, spleen, umbilical cord (1 cm from near site on insertion). Place in sterile yellow lid container. Add sterile saline if transport is likely to be delayed. | ||
Note: these samples must be unfixed | Note: these samples must be unfixed | ||
== Frozen Sections == | == Frozen Sections == | ||
The Histology department needs to be notified <u>before</u> a frozen section sample | The Histology department needs to be notified <u>before</u> a frozen section sample is to be done. The requesting clinician needs to discuss the case with the pathologist directly before proceeding. Contact 2636 to be referred to the relevant pathologist. Inform the pathologist what time the sample is expected to be taken. | ||
The sample needs to be sent '''urgently''' with an orderly directly to the histology department, ensure this does not go to the main laboratory reception. Phone Histology on 2636 when the specimen is on its way to the laboratory. | The sample needs to be sent '''urgently''' with an orderly directly to the histology department, ensure this does not go to the main laboratory reception. Phone Histology on 2636 when the specimen is on its way to the laboratory. | ||
== Immunofluorescence == | == Immunofluorescence == | ||
If Immunofluorescence studies are required a separate tissue portion should be put into Michel's medium (available on request from the histology department) and a separate biopsy | If Immunofluorescence studies are required, a separate tissue portion should be put into Michel's medium (available on request from the histology department) and a separate biopsy is to be submitted in formalin. | ||
The samples ideally should be taken from the same site. | The samples ideally should be taken from the same site. | ||
Immunofluorescence samples are processed by Medlab Central in Palmerston North. Histology is responsible for transporting the specimen. | Immunofluorescence samples are processed by Medlab Central in Palmerston North. Histology is responsible for transporting the specimen. Reports will show in clinical portal once complete. | ||
== Lymphoma/Surface Markers == | == Lymphoma/Surface Markers == | ||
Please notify the histology laboratory that a procedure is planned for a fresh lymph node biopsy for query lymphoma. This needs to be discussed with the relevant pathologist before proceeding. | '''Lymph Node Biopsy''': Please notify the histology laboratory that a procedure is planned for a fresh lymph node biopsy for query lymphoma/flow cytometry/surface markers. This needs to be discussed with the relevant pathologist before proceeding. | ||
The sample needs to be sent urgently via an orderly, in a sterile container with no media added, to the histology laboratory. If sample is also for microbiology, please clearly write this on the request form. | The sample needs to be sent urgently via an orderly, in a sterile container with no media added, to the histology laboratory. If sample is also for microbiology, please clearly write this on the request form. | ||
The sample for surface marker studies is sent to Canterbury Health Laboratories for processing. The report will show in clinical portal once complete. The remaining tissue is submitted for routine histology and processed in-house. | |||
Phone the laboratory on EXT 2684 when the sample has been sent. | Phone the laboratory on EXT 2684 when the sample has been sent. | ||
== Placenta/Foetus == | '''FNA/Core Biopsy''': RPMI Transport media can be requested from the lab. Biopsy can be placed directly into RPMI at collection. Ensure either a cytology or histology sample accompanies the RPMI sample as these results are interpreted together. | ||
'''Placenta:''' | |||
== Placenta/Foetus/Products of Conception == | |||
'''Products of Conception:''' | |||
Products of conception/ERPOC under 12 weeks. This is processed as routine histology and completed in house. Sample is to be placed into Formalin. If the sample is fresh, send the sample immediately to the laboratory. | |||
If cytogenetics testing is required, a separate sample is to be taken and placed in a sterile container with a separate request form stating "Cytogenetics testing". Please see "Cytogenetics" above for further details.'''Placenta:''' | |||
Placentas are received into Histology and sent to Wellington SCL for processing. If the sample is fresh, send the sample immediately to the laboratory. | Placentas are received into Histology and sent to Wellington SCL for processing. If the sample is fresh, send the sample immediately to the laboratory. | ||
If cytogenetics testing is required a separate sample is to be taken and placed in a sterile container with a separate request form stating "Cytogenetics testing". Please see "Cytogenetics" above for further details. | If cytogenetics testing is required, a separate sample is to be taken and placed in a sterile container with a separate request form stating "Cytogenetics testing". Please see "Cytogenetics" above for further details. | ||
'''Foetus:''' | '''Foetus:''' | ||
A foetus that is requiring post mortem and is between 12-20 weeks | A foetus that is requiring post mortem and is between 12-20 weeks is received into Histology and sent to Wellington SCL for processing. | ||
A foetus over 20 weeks is not accepted into the laboratory. | A foetus over 20 weeks is not accepted into the laboratory and transport is arranged with maternity. | ||
If cytogenetics testing is required a separate sample is to be taken and placed in a sterile container with a separate request form stating "Cytogenetics testing". Please see "Cytogenetics" above for further details. | If cytogenetics testing is required, a separate sample is to be taken and placed in a sterile container with a separate request form stating "Cytogenetics testing". Please see "Cytogenetics" above for further details. | ||
If the patient wishes to have any tissue returned after processing please ensure a ''Body Parts / Bodily Substances Return Form - Section 1'' has been filled in. See Tissue Return to Patient Service below for further details. | If the patient wishes to have any tissue returned after processing please ensure a ''Body Parts / Bodily Substances Return Form - Section 1'' has been filled in. See Tissue Return to Patient Service below for further details. | ||