Histology Department Information - Revision history

AKernaghan: /* Results & General Enquiries */

2025-01-21T21:26:36Z

Results & General Enquiries

← Older revision		Revision as of 21:26, 21 January 2025
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	'''Email:''' histology@hbdhb.govt.nz		'''Email:''' histology@hbdhb.govt.nz

	'''Head of Department:''' ~~Maureen Ali~~ 06 878 1308 Ext 2485		'''Head of Department:''' Jade Chetty 06 878 1308 Ext 2485

Abaylis: /* Results & General Enquiries */

2022-09-11T21:41:23Z

Results & General Enquiries

← Older revision		Revision as of 21:41, 11 September 2022
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	'''For technical queries/laboratory:''' Ext 2636 or 2684		'''For technical queries/laboratory:''' 06 878 1308 Ext 2636 or 2684

	'''Admin and reports:''' Ext 2644		'''Admin and reports:''' Ext 2644
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	'''Email:''' histology@hbdhb.govt.nz		'''Email:''' histology@hbdhb.govt.nz

	'''Head of Department:''' Maureen Ali Ext 2485		'''Head of Department:''' Maureen Ali 06 878 1308 Ext 2485


	'''Pathologists:'''		'''Pathologists:'''

	'''Dr Lesley Joblin''' Ext 2647		'''Dr Lesley Joblin''' 06 878 1308 Ext 2647

	'''Dr David Palmer''' Ext 2599		'''Dr David Palmer''' 06 878 1308 Ext 2599

	'''Dr Adrian Ormsby''' Ext 2685		'''Dr Adrian Ormsby''' 06 878 1308 Ext 2685

Abaylis at 02:47, 9 September 2022

2022-09-09T02:47:55Z

← Older revision		Revision as of 02:47, 9 September 2022
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	'''<big>Anatomical Histopathology operates Monday to Friday 0800-1700</big>'''		'''<big>Anatomical Histopathology operates Monday to Friday 0800-1700</big>'''

			Process all routine histology, immunohistochemistry, and frozen sectioning in-house. Specialized testing is sent to external laboratories as indicated below.

	== Histology ==		== Histology ==
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	If a sample is required to be split for testing, ensure all tests are written on the request from clearly. Once CytoRich Red has been added to a sample, no other testing can be completed.		If a sample is required to be split for testing, ensure all tests are written on the request from clearly. Once CytoRich Red has been added to a sample, no other testing can be completed.

	Contact SCL Dunedin for enquiries and results for cytology specimens 03 477 6981		Contact SCL Dunedin for enquiries and results for cytology specimens: 03 477 6981

	== Cytogenetics ==		== Cytogenetics ==
	Cytogenetics testing is completed by Wellington Regional Genetics Laboratory. WRGL offers tissue culture, karyotyping, QF-PCR, DNA extraction and frozen sample storage for postnatal and post mortem tissue samples where chromosomal or genetic abnormalities are suspected (http://www.wellingtongenetics.co.nz/). Hastings Hospital laboratory is responsible for transporting the specimen to WRGL.		Cytogenetics testing is completed by Wellington Regional Genetics Laboratory. WRGL offers tissue culture, karyotyping, QF-PCR, DNA extraction and frozen sample storage for postnatal and post mortem tissue samples where chromosomal or genetic abnormalities are suspected (http://www.wellingtongenetics.co.nz/). Hastings Hospital laboratory is responsible for transporting the specimen to WRGL.

	For any sample requiring cytogenetic studies please clearly write on the request form the gestational age, site of biopsy, and "Cytogenetics Testing". Send the sample immediately to the laboratory. If the sample is also requiring routine histology, ensure a sample for cytogenetics is taken <u>before</u> any formalin is added. If further information is required please contact the Histology department or contact Wellington Regional Genetics Laboratory 04 918 6967.		For any sample requiring cytogenetic studies please clearly write on the request form the gestational age, site of biopsy, and "Cytogenetics Testing". Send the sample immediately to the laboratory. If the sample is also requiring routine histology, ensure the sample for cytogenetics is taken <u>before</u> any formalin is added. Send both samples together to the laboratory.

			If further information is required please contact the Histology department (EXT 2636) or contact Wellington Regional Genetics Laboratory 04 918 6967.

	'''The laboratory staff are not responsible for taking a biopsy for cytogenetics. This is to be completed by the requesting clinician.'''		'''The laboratory staff are not responsible for taking a biopsy for cytogenetics. This is to be completed by the requesting clinician.'''
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	'''For Tissue Karyotype/Cytogenetic studies sample requirements are:'''		'''For Tissue Karyotype/Cytogenetic studies sample requirements are:'''

	'''Miscarriage (<20 weeks)''': Products of Conception including Chorionic villi, fetal limb, umbilical cord, collected in a sterile yellow lid container. Add sterile saline if transport is likely to delayed.		'''Miscarriage (<20 weeks)''': Products of Conception including Chorionic villi, fetal limb, umbilical cord, collected in a sterile yellow lid container. Add sterile saline if transport is likely to be delayed.



	'''IUD, Stillbirth, perinatal death (>20weeks)''', '''Other:'''		'''IUD, Stillbirth, perinatal death (>20weeks)''', '''Other:'''

	Skin – 1 cm full thickness, cartilage, spleen, umbilical cord (1 cm from near site on insertion). Place in sterile yellow lid container. Add sterile saline if transport is likely to delayed.		Skin – 1 cm full thickness, cartilage, spleen, umbilical cord (1 cm from near site on insertion). Place in sterile yellow lid container. Add sterile saline if transport is likely to be delayed.

	Note: these samples must be unfixed		Note: these samples must be unfixed

	== Frozen Sections ==		== Frozen Sections ==
	The Histology department needs to be notified <u>before</u> a frozen section sample ~~can~~ be ~~sent up~~. The requesting clinician needs to discuss the case with the pathologist directly before proceeding. Contact 2636 to be referred to the relevant pathologist. Inform the pathologist what time the sample is expected to be taken.		The Histology department needs to be notified <u>before</u> a frozen section sample is to be done. The requesting clinician needs to discuss the case with the pathologist directly before proceeding. Contact 2636 to be referred to the relevant pathologist. Inform the pathologist what time the sample is expected to be taken.

	The sample needs to be sent '''urgently''' with an orderly directly to the histology department, ensure this does not go to the main laboratory reception. Phone Histology on 2636 when the specimen is on its way to the laboratory.		The sample needs to be sent '''urgently''' with an orderly directly to the histology department, ensure this does not go to the main laboratory reception. Phone Histology on 2636 when the specimen is on its way to the laboratory.

	== Immunofluorescence ==		== Immunofluorescence ==
	If Immunofluorescence studies are required a separate tissue portion should be put into Michel's medium (available on request from the histology department) and a separate biopsy ~~needs~~ to be submitted in formalin.		If Immunofluorescence studies are required, a separate tissue portion should be put into Michel's medium (available on request from the histology department) and a separate biopsy is to be submitted in formalin.

	The samples ideally should be taken from the same site.		The samples ideally should be taken from the same site.

	Immunofluorescence samples are processed by Medlab Central in Palmerston North. Histology is responsible for transporting the specimen.		Immunofluorescence samples are processed by Medlab Central in Palmerston North. Histology is responsible for transporting the specimen. Reports will show in clinical portal once complete.

	== Lymphoma/Surface Markers ==		== Lymphoma/Surface Markers ==
	Please notify the histology laboratory that a procedure is planned for a fresh lymph node biopsy for query lymphoma. This needs to be discussed with the relevant pathologist before proceeding.		'''Lymph Node Biopsy''': Please notify the histology laboratory that a procedure is planned for a fresh lymph node biopsy for query lymphoma/flow cytometry/surface markers. This needs to be discussed with the relevant pathologist before proceeding.

	The sample needs to be sent urgently via an orderly, in a sterile container with no media added, to the histology laboratory. If sample is also for microbiology, please clearly write this on the request form.		The sample needs to be sent urgently via an orderly, in a sterile container with no media added, to the histology laboratory. If sample is also for microbiology, please clearly write this on the request form.

			The sample for surface marker studies is sent to Canterbury Health Laboratories for processing. The report will show in clinical portal once complete. The remaining tissue is submitted for routine histology and processed in-house.

	Phone the laboratory on EXT 2684 when the sample has been sent.		Phone the laboratory on EXT 2684 when the sample has been sent.

	== Placenta/Foetus ==		'''FNA/Core Biopsy''': RPMI Transport media can be requested from the lab. Biopsy can be placed directly into RPMI at collection. Ensure either a cytology or histology sample accompanies the RPMI sample as these results are interpreted together.
	'''Placenta:'''
			== Placenta/Foetus/Products of Conception ==
			'''Products of Conception:'''

			Products of conception/ERPOC under 12 weeks. This is processed as routine histology and completed in house. Sample is to be placed into Formalin. If the sample is fresh, send the sample immediately to the laboratory.

			If cytogenetics testing is required, a separate sample is to be taken and placed in a sterile container with a separate request form stating "Cytogenetics testing". Please see "Cytogenetics" above for further details.'''Placenta:'''

	Placentas are received into Histology and sent to Wellington SCL for processing. If the sample is fresh, send the sample immediately to the laboratory.		Placentas are received into Histology and sent to Wellington SCL for processing. If the sample is fresh, send the sample immediately to the laboratory.

	If cytogenetics testing is required a separate sample is to be taken and placed in a sterile container with a separate request form stating "Cytogenetics testing". Please see "Cytogenetics" above for further details.		If cytogenetics testing is required, a separate sample is to be taken and placed in a sterile container with a separate request form stating "Cytogenetics testing". Please see "Cytogenetics" above for further details.

	'''Foetus:'''		'''Foetus:'''

	A foetus that is requiring post mortem and is between 12-20 weeks ~~are~~ received into Histology and sent to Wellington SCL for processing.		A foetus that is requiring post mortem and is between 12-20 weeks is received into Histology and sent to Wellington SCL for processing.

	A foetus over 20 weeks is not accepted into the laboratory.		A foetus over 20 weeks is not accepted into the laboratory and transport is arranged with maternity.

	If cytogenetics testing is required a separate sample is to be taken and placed in a sterile container with a separate request form stating "Cytogenetics testing". Please see "Cytogenetics" above for further details.		If cytogenetics testing is required, a separate sample is to be taken and placed in a sterile container with a separate request form stating "Cytogenetics testing". Please see "Cytogenetics" above for further details.

	If the patient wishes to have any tissue returned after processing please ensure a ''Body Parts / Bodily Substances Return Form - Section 1'' has been filled in. See Tissue Return to Patient Service below for further details.		If the patient wishes to have any tissue returned after processing please ensure a ''Body Parts / Bodily Substances Return Form - Section 1'' has been filled in. See Tissue Return to Patient Service below for further details.

Abaylis: /* Placenta/Foetus */

2022-09-09T02:28:43Z

Placenta/Foetus

← Older revision		Revision as of 02:28, 9 September 2022
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	'''Placenta:'''		'''Placenta:'''

	Placentas are received into Histology and sent to Wellington SCL for processing. If the sample is ~~sent~~ fresh, send the sample immediately to the laboratory.		Placentas are received into Histology and sent to Wellington SCL for processing. If the sample is fresh, send the sample immediately to the laboratory.

	If cytogenetics testing is required a separate sample is to be taken and placed in a sterile container with a separate request form stating "Cytogenetics testing". Please see "Cytogenetics" above for further details.		If cytogenetics testing is required a separate sample is to be taken and placed in a sterile container with a separate request form stating "Cytogenetics testing". Please see "Cytogenetics" above for further details.
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	A foetus over 20 weeks is not accepted into the laboratory.		A foetus over 20 weeks is not accepted into the laboratory.

			If cytogenetics testing is required a separate sample is to be taken and placed in a sterile container with a separate request form stating "Cytogenetics testing". Please see "Cytogenetics" above for further details.

	If the patient wishes to have any tissue returned after processing please ensure a ''Body Parts / Bodily Substances Return Form - Section 1'' has been filled in. See Tissue Return to Patient Service below for further details.		If the patient wishes to have any tissue returned after processing please ensure a ''Body Parts / Bodily Substances Return Form - Section 1'' has been filled in. See Tissue Return to Patient Service below for further details.

Abaylis: /* Placenta/Foetus */

2022-09-09T00:57:35Z

Placenta/Foetus

← Older revision		Revision as of 00:57, 9 September 2022
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	== Placenta/Foetus ==		== Placenta/Foetus ==
			'''Placenta:'''

			Placentas are received into Histology and sent to Wellington SCL for processing. If the sample is sent fresh, send the sample immediately to the laboratory.

			If cytogenetics testing is required a separate sample is to be taken and placed in a sterile container with a separate request form stating "Cytogenetics testing". Please see "Cytogenetics" above for further details.

			'''Foetus:'''

			A foetus that is requiring post mortem and is between 12-20 weeks are received into Histology and sent to Wellington SCL for processing.

			A foetus over 20 weeks is not accepted into the laboratory.

			If the patient wishes to have any tissue returned after processing please ensure a ''Body Parts / Bodily Substances Return Form - Section 1'' has been filled in. See Tissue Return to Patient Service below for further details.

	== Muscle Biopsy ==		== Muscle Biopsy ==

Abaylis: /* Lymphoma/Surface Markers */

2022-09-08T22:20:11Z

Lymphoma/Surface Markers

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 == Lymphoma/Surface Markers ==
 == Placenta/Foetus ==
 == Tissue Return To Patient Services (RTP) ==

Abaylis: /* Cytology */

2022-09-07T22:35:32Z

Cytology

← Older revision		Revision as of 22:35, 7 September 2022
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	* For return to patient service, see below.		* For return to patient service, see below.
	* Cytology and Gynae specimens are processed by SCL Dunedin. Please contact SCL Dunedin for results 03 477 6981		* Cytology and Gynae specimens are processed by SCL Dunedin. Please contact SCL Dunedin for results 03 477 6981
			[[File:Formalin Hazard Labels.png\|thumb\|336x336px\|Formalin Hazard Labels]]
	'''Formalin is a known carcinogenic, and must be handled with appropriate PPE. Nitrile gloves, safety glasses/face shield, gown and in a well ventilated area.'''		'''Formalin is a known carcinogenic, and must be handled with appropriate PPE. Nitrile gloves, safety glasses/face shield, gown and in a well ventilated area.'''

	Acutely toxic, irritant to skin, eyes, respiratory, known carcinogen and mutagen, causes damage to organs. See SDS for further information. ~~If there is a formalin spill contact *7777 and evacuate the area immediately.~~		Acutely toxic, irritant to skin, eyes, respiratory, known carcinogen and mutagen, causes damage to organs. See SDS for further information.
	~~[[File:Formalin Hazard Labels.png\|thumb\|336x336px\|Formalin Hazard Labels]]~~

			If there is a formalin spill contact *7777 and evacuate the area immediately.

			== Cytology ==
			Cytology samples are to be sent fresh and urgently to the laboratory. All cytology samples are processed by SCL Dunedin. Histology is responsible for the addition of CytoRich Red and transporting the specimen.

	~~== Cytology ==~~		If a sample is required to be split for testing, ensure all tests are written on the request from clearly. Once CytoRich Red has been added to a sample, no other testing can be completed.

			Contact SCL Dunedin for enquiries and results for cytology specimens 03 477 6981

	== Cytogenetics ==		== Cytogenetics ==

Abaylis: /* Results & General Enquiries */

2022-09-07T22:29:22Z

Results & General Enquiries

← Older revision		Revision as of 22:29, 7 September 2022
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	== Immunofluorescence ==		== Immunofluorescence ==
			If Immunofluorescence studies are required a separate tissue portion should be put into Michel's medium (available on request from the histology department) and a separate biopsy needs to be submitted in formalin.

			The samples ideally should be taken from the same site.

			Immunofluorescence samples are processed by Medlab Central in Palmerston North. Histology is responsible for transporting the specimen.

	== Lymphoma/Surface Markers ==		== Lymphoma/Surface Markers ==
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	== Results & General Enquiries ==		== Results & General Enquiries ==
	Results are not given out over the phone. Please contact the Admin team.		Results are not given out over the phone. Please contact the Admin team for results.

Abaylis: /* Frozen Sections */

2022-09-07T21:38:02Z

Frozen Sections

← Older revision		Revision as of 21:38, 7 September 2022
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	== Histology ==		== Histology ==
	Anatomical Pathology receives Histology specimens from both hospital and community patients for the entire Hawkes Bay region. ~~Provide~~ Histology services for National Bowel Screening Program. Routine Histology testing is performed onsite.		Anatomical Pathology receives Histology specimens from both hospital and community patients for the entire Hawkes Bay region. Histology services are also provided for the National Bowel Screening Program. Routine Histology testing is performed onsite.

	* Samples and request forms need to be labelled with patient name, NHI, and DOB. Request forms must state specimen type and relevant clinical details for reason of testing. Failure to provide these details may result in delayed processing of ~~specimen~~. The requesting clinician must sign the request form. Please ensure current up to date labels are used on the request forms to ensure results are reported to the correct clinician.		* Samples and request forms need to be labelled with patient name, NHI, and DOB. Request forms must state specimen type, specimen site and relevant clinical details for reason of testing. Failure to provide these details may result in delayed processing of specimens. The requesting clinician must sign the request form. Please ensure current up to date BRADMA labels are used on the request forms to ensure results are reported to the correct clinician.
	* Specimens for routine histology should be sent to the laboratory in a large enough container to hold '''<u>at least 5 times the volume of the specimen, 5:1 ratio of formalin to specimen.</u>''' The container needs to be labelled with all patient details and site of specimen. Specimen containers are to be tightly sealed to avoid leakages.		* Specimens for routine histology should be sent to the laboratory in a large enough container to hold '''<u>at least 5 times the volume of the specimen, 5:1 ratio of formalin to specimen.</u>''' The container needs to be labelled with all patient details and site of specimen. Specimen containers are to be tightly sealed to avoid leakages.
	* For return to patient service, see below		* For return to patient service, see below.
			* Cytology and Gynae specimens are processed by SCL Dunedin. Please contact SCL Dunedin for results 03 477 6981
	'''Formalin is a known carcinogenic, and must be handled with appropriate PPE. Nitrile gloves, safety glasses/face shield, gown and in a well ventilated area.'''		'''Formalin is a known carcinogenic, and must be handled with appropriate PPE. Nitrile gloves, safety glasses/face shield, gown and in a well ventilated area.'''

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	Cytogenetics testing is completed by Wellington Regional Genetics Laboratory. WRGL offers tissue culture, karyotyping, QF-PCR, DNA extraction and frozen sample storage for postnatal and post mortem tissue samples where chromosomal or genetic abnormalities are suspected (http://www.wellingtongenetics.co.nz/). Hastings Hospital laboratory is responsible for transporting the specimen to WRGL.		Cytogenetics testing is completed by Wellington Regional Genetics Laboratory. WRGL offers tissue culture, karyotyping, QF-PCR, DNA extraction and frozen sample storage for postnatal and post mortem tissue samples where chromosomal or genetic abnormalities are suspected (http://www.wellingtongenetics.co.nz/). Hastings Hospital laboratory is responsible for transporting the specimen to WRGL.

			For any sample requiring cytogenetic studies please clearly write on the request form the gestational age, site of biopsy, and "Cytogenetics Testing". Send the sample immediately to the laboratory. If the sample is also requiring routine histology, ensure a sample for cytogenetics is taken <u>before</u> any formalin is added. If further information is required please contact the Histology department or contact Wellington Regional Genetics Laboratory 04 918 6967.

			'''The laboratory staff are not responsible for taking a biopsy for cytogenetics. This is to be completed by the requesting clinician.'''

	'''For Tissue Karyotype/Cytogenetic studies sample requirements are:'''		'''For Tissue Karyotype/Cytogenetic studies sample requirements are:'''

	'''Miscarriage (<20 weeks)''': Products of Conception including Chorionic villi, fetal limb, umbilical cord, collected in a sterile container. Add sterile saline if transport is likely to delayed.		'''Miscarriage (<20 weeks)''': Products of Conception including Chorionic villi, fetal limb, umbilical cord, collected in a sterile yellow lid container. Add sterile saline if transport is likely to delayed.




	'''IUD, Stillbirth, perinatal death (>20weeks)''', '''Other:'''		'''IUD, Stillbirth, perinatal death (>20weeks)''', '''Other:'''

	Skin – 1 cm full thickness, cartilage, spleen, umbilical cord (1 cm from near site on insertion). Place in sterile container. Add sterile saline if transport is likely to delayed.		Skin – 1 cm full thickness, cartilage, spleen, umbilical cord (1 cm from near site on insertion). Place in sterile yellow lid container. Add sterile saline if transport is likely to delayed.

	Note: these samples must be unfixed		Note: these samples must be unfixed

	== Frozen Sections ==		== Frozen Sections ==
	The Histology department needs to be notified before a frozen section sample can be sent up. The requesting clinician needs to discuss the case with the pathologist directly before proceeding. Contact 2636 to be referred to the relevant pathologist.		The Histology department needs to be notified <u>before</u> a frozen section sample can be sent up. The requesting clinician needs to discuss the case with the pathologist directly before proceeding. Contact 2636 to be referred to the relevant pathologist. Inform the pathologist what time the sample is expected to be taken.

	~~Notify~~ the ~~laboratory when~~ the sample is to be taken ~~and what time it is expected in the laboratory~~.

	The sample needs to be sent urgently with an orderly directly to the histology department, ensure this does not go to the main laboratory reception.		The sample needs to be sent '''urgently''' with an orderly directly to the histology department, ensure this does not go to the main laboratory reception. Phone Histology on 2636 when the specimen is on its way to the laboratory.

	== Immunofluorescence ==		== Immunofluorescence ==
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	== Placenta/Foetus ==		== Placenta/Foetus ==

	== RTP ==		== Tissue Return To Patient Services (RTP) ==
	For any sample requiring return to patient services.		For any sample requiring return to patient services, Ensure the specimen container and request form is labelled with Return to Patient red stickers. Fill in the ''Body Parts / Bodily Substances Return Form - Section 1.''

	~~Ensure~~ the ~~specimen container and~~ request form ~~is labelled~~ with ~~Return to Patient red stickers. Fill in~~ the ''Body Parts / Bodily Substances Return Form ~~- Section 1~~.''		Send the filled in request form with the Body Parts / Bodily Substances Return Form and specimen to the laboratory for processing.

	~~Send filled in return form with specimen and request form~~ to the laboratory.		Patient is to contact the laboratory 7 days after the sample has been processed to arrange a date/time for collection.

	== Results & General Enquiries ==		== Results & General Enquiries ==

Abaylis: /* Cytogenetics */

2022-09-06T01:50:17Z

Cytogenetics

← Older revision		Revision as of 01:50, 6 September 2022
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	== Cytogenetics ==		== Cytogenetics ==
	Cytogenetics testing is completed by Wellington Regional Genetics Laboratory. WRGL offers tissue culture, karyotyping, QF-PCR, DNA extraction and frozen sample storage for postnatal and post mortem tissue samples where chromosomal or genetic abnormalities are suspected (http://www.wellingtongenetics.co.nz/).		Cytogenetics testing is completed by Wellington Regional Genetics Laboratory. WRGL offers tissue culture, karyotyping, QF-PCR, DNA extraction and frozen sample storage for postnatal and post mortem tissue samples where chromosomal or genetic abnormalities are suspected (http://www.wellingtongenetics.co.nz/). Hastings Hospital laboratory is responsible for transporting the specimen to WRGL.


	For Tissue Karyotype/Cytogenetic studies sample requirements are:
			'''For Tissue Karyotype/Cytogenetic studies sample requirements are:'''

	'''Miscarriage (<20 weeks)''': Products of Conception including Chorionic villi, fetal limb, umbilical cord, collected in a sterile container. Add sterile saline if transport is likely to delayed.		'''Miscarriage (<20 weeks)''': Products of Conception including Chorionic villi, fetal limb, umbilical cord, collected in a sterile container. Add sterile saline if transport is likely to delayed.